| Autor: |
Timmermans, Sjoerd A. M. E. G., Damoiseaux, Jan G. M. C., Heerings-Rewinkel, Petra T. J., Ayalon, Rivka, Beck Jr, Laurence H., Schlumberger, Wolfgang, Salant, David J., van Paassen, Pieter, Tervaert, Jan Willem Cohen |
| Předmět: |
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| Zdroj: |
American Journal of Clinical Pathology; Jul2014, Vol. 142 Issue 1, p29-34, 6p, 4 Charts, 1 Graph |
| Abstrakt: |
Objectives: Autoantibodies against the M-type phospholipase A2 receptor 1 (anti-PLA2R1) have been demonstrated to be very specific for idiopathic membranous nephropathy (MN). We studied a novel enzyme-linked immunosorbent assay (ELISA) and compared results with results obtained using an indirect immunofluorescence (IIF) and a Western blotting test (WB). Methods: One-hundred nine patients with idiopathic MN were recruited between November 1979 and March 2011. The control cohort comprised serum samples from patients with secondary MN (n = 16) and nephrotic controls (n = 17). The presence of anti-PLA2R1 in serum samples obtained at the time of renal biopsy was determined using ELISA, IIF, and WB. Results: With similar specificity (≥ 97%), sensitivity varied from 68% (IIF) to 72% (ELISA, WB). Remarkably, patients who were seronegative for anti-PLA2R1 more often entered spontaneous remission (P = .038), whereas seropositive patients were more frequently treated with immunosuppressive agents (P < .001). Conclusions: ELISA performs excellently in differentiating idiopathic from secondary MN. Furthermore, ELISA shared high agreement with WB and IIF. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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