Two-step inhibitory effect of kanzo on oxytocin-induced and prostaglandin F-induced uterine myometrial contractions.

Autor: Sumi, Genichiro, Yasuda, Katsuhiko, Kanamori, Chiharu, Kajimoto, Megumi, Nishigaki, Akemi, Tsuzuki, Tomoko, Cho, Hisayuu, Okada, Hidetaka, Kanzaki, Hideharu
Zdroj: Journal of Natural Medicines; Jul2014, Vol. 68 Issue 3, p550-560, 11p
Abstrakt: We previously reported that shakuyaku-kanzo-to, a kampo medicine consisting of shakuyaku and kanzo, has an inhibitory effect on myometrial contractions in pregnant women. In this study, we evaluated the effects of kanzo, glycyrrhizin (a major component of kanzo), glycyrrhetinic acid (GA; a major metabolite of glycyrrhizin), shakuyaku, and paeoniflorin (a major component of shakuyaku) on agonist-induced contractions of the uterus of pregnant humans and rats. We prepared myometrial strips from the uterus of pregnant humans and rats and induced contractions with oxytocin (50 μU/mL) or prostaglandin F (PGF) (10 or 10 M). Kanzo (250 μg/mL) and GA (5 × 10 M) inhibited the oxytocin-induced and PGF-induced contractions in pregnant human and rat myometrium, but shakuyaku (250 μg/mL), paeoniflorin (10 M), and glycyrrhizin (10 M) did not inhibit contractions in either. Interestingly, kanzo and GA showed an inhibitory effect after temporarily enhancing the PGF-induced contractions in the rat myometrium, but not in the human myometrium. These results suggest that kanzo has at least a two-step inhibitory effect on the myometrial contractions that originate from the kanzo itself and a metabolite of glycyrrhizin in kanzo. Furthermore, kanzo was found to be safe for inhibiting PGF-induced contractions in humans because it did not temporarily enhance PGF-induced contractions. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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