| Autor: |
Sharp, Christine E., Smirnova, Angela V., Graham, Jaime M., Stott, Matthew B., Khadka, Roshan, Moore, Tim R., Grasby, Stephen E., Strack, Maria, Dunfield, Peter F. |
| Předmět: |
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| Zdroj: |
Environmental Microbiology; Jun2014, Vol. 16 Issue 6, p1867-1878, 12p |
| Abstrakt: |
Recently, methanotrophic members of the phylum V errucomicrobia have been described, but little is known about their distribution in nature. We surveyed methanotrophic bacteria in geothermal springs and acidic wetlands via pyrosequencing of 16 S rRNA gene amplicons. Putative methanotrophic V errucomicrobia were found in samples covering a broad temperature range (22.5-81.6° C), but only in acidic conditions ( pH 1.8-5.0) and only in geothermal environments, not in acidic bogs or fens. Phylogenetically, three 16 S rRNA gene sequence clusters of putative methanotrophic V errucomicrobia were observed. Those detected in high-temperature geothermal samples (44.1-81.6° C) grouped with known thermoacidiphilic ' M ethylacidiphilum' isolates. A second group dominated in moderate-temperature geothermal samples (22.5-40.1° C) and a representative mesophilic methanotroph from this group was isolated (strain LP2 A). Genome sequencing verified that strain LP2 A possessed particulate methane monooxygenase, but its 16 S rRNA gene sequence identity to ' M ethylacidiphilum infernorum' strain V4 was only 90.6%. A third group clustered distantly with known methanotrophic V errucomicrobia. Using pmoA-gene targeted quantitative polymerase chain reaction, two geothermal soil profiles showed a dominance of LP2 A-like pmoA sequences in the cooler surface layers and ' M ethylacidiphilum'-like pmoA sequences in deeper, hotter layers. Based on these results, there appears to be a thermophilic group and a mesophilic group of methanotrophic V errucomicrobia. However, both were detected only in acidic geothermal environments. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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