Mutants of phospholipase A ( pPLA- I) have a red light and auxin phenotype.

Autor: EFFENDI, YUNUS, RADATZ, KATRIN, LABUSCH, CORINNA, RIETZ, STEFFEN, WIMALASEKERA, RINUKSHI, HELIZON, HANNA, ZEIDLER, MATHIAS, SCHERER, GÜNTHER F. E.
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Zdroj: Plant, Cell & Environment; Jul2014, Vol. 37 Issue 7, p1626-1640, 15p
Abstrakt: pPLA-I is the evolutionarily oldest patatin-related phospholipase A ( pPLA) in plants, which have previously been implicated to function in auxin and defence signalling. Molecular and physiological analysis of two allelic null mutants for pPLA-I [ ppla-I-1 in Wassilewskija ( Ws) and ppla-I-3 in Columbia ( Col) ] revealed pPLA-I functions in auxin and light signalling. The enzyme is localized in the cytosol and to membranes. After auxin application expression of early auxin-induced genes is significantly slower compared with wild type and both alleles show a slower gravitropic response of hypocotyls, indicating compromised auxin signalling. Additionally, phytochrome-modulated responses like abrogation of gravitropism, enhancement of phototropism and growth in far red-enriched light are decreased in both alleles. While early flowering, root coils and delayed phototropism are only observed in the Ws mutant devoid of phyD, the light-related phenotypes observed in both alleles point to an involvement of pPLA-I in phytochrome signalling. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index