Three-Dimensional Protein Networks Assembled by Two-Photon Activation.

Autor: Gatterdam, Volker, Ramadass, Radhan, Stoess, Tatjana, Fichte, Manuela A. H., Wachtveitl, Josef, Heckel, Alexander, Tampé, Robert
Předmět:
Zdroj: Angewandte Chemie International Edition; May2014, Vol. 53 Issue 22, p5680-5684, 5p
Abstrakt: Spatial and temporal control over chemical and biological processes plays a key role in life and material sciences. Here we synthesized a two-photon-activatable glutathione (GSH) to trigger the interaction with glutathione S-transferase (GST) by light at superior spatiotemporal resolution. The compound shows fast and well-confined photoconversion into the bioactive GSH, which is free to interact with GST-tagged proteins. The GSH/GST interaction can be phototriggered, changing its affinity over several orders of magnitude into the nanomolar range. Multiplexed three-dimensional (3D) protein networks are simultaneously generated in situ through two-photon fs-pulsed laser-scanning excitation. The two-photon activation facilitates the three-dimensional assembly of protein structures in real time at hitherto unseen resolution in time and space, thus opening up new applications far beyond the presented examples. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index