| Autor: |
Carus, Andreas, Donskov, Frede, Nielsen, Patricia S., Hager, Henrik, Nedergaard, Bettina S., Steiniche, Torben, Ladekarl, Morten |
| Předmět: |
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| Zdroj: |
Journal of OncoPathology; Jun2014, Vol. 2 Issue 2, p1-9, 9p |
| Abstrakt: |
INTRODUCTION Manual observer-assisted stereological (OAS) assessments of tumor-infiltrating neutrophils and lymphocytes are prognostic, accurate, but cumbersome. We assessed the applicability of automated digital image analysis (DIA). METHODS Visiomorph software was used to obtain DIA densities of immunostains for CD66b+ neutrophils, CD163+ macrophages, and CD8+ lymphocytes in tumors from 101 patients with International Federation of Gynecology and Obstetrics (FIGO) stage IB/IIA cervical cancer. Results were compared with manual OAS assessments. RESULTS Automated DIA assessment was faster and required less human resources than manual OAS assessments. We observed high correlations between DIA and OAS variables forCD8+ lymphocytes, CD66b+ neutrophils, and CD163+ macrophages (spearman ? > 0.8; P < 0.0001). Hazard rates for recurrence of DIA assessments in the global tumor area were comparable with the prognostically strongest manual OAS assessments in the peritumoral compartment. In multivariate analysis, CD66b and CD8 densities, assessed by DIA, and regional lymph node metastases were independent predictors of RFS, while CD163 density and FIGO stage were not. The CD66b/CD8 tumorassociated neutrophil to lymphocyte (TA-NL) index accurately predicted the risk of relapse, ranging from 8% to 52% (P 0.001). CONCLUSIONS DIA is a potential assessment technique. The TA-NL index obtained by DIA is a strong prognostic variable with possible routine clinical application. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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