Purification and Characterization of Mouse Single-Chain Antibody against Polycyclic Aromatic Hydrocarbons.

Autor:	Ustinov, Valentin A., Averjanov, Anton V., Glushkov, Andrey N.
Předmět:	MONOCLONAL antibodies POLYCYCLIC aromatic hydrocarbons IMMUNOLOGY LABORATORY mice CELLULOSE LUNG cancer treatment
Zdroj:	Journal of Immunoassay & Immunochemistry; Oct2014, Vol. 35 Issue 4, p428-441, 14p
Abstrakt:	Polycyclic aromatic hydrocarbons (PAH) such as benzo[a]pyren mainly induce lung cancer in humans. We characterized the mouse single chain antibody against benzo[a]pyren (pSh). pSh was expressed and purified as cellulose binding domain fusion (pSh-CBD). The pSh-CBD bound five different PAH with high affinity. The 18 amino acid linker connected pSh-CBD heavy and light chains provided correct protein folding. The KDs for pSh-CBD and polycyclic aromatic hydrocarbons were similar to KDs for monoclonal antibody, approximately 10−8. Separately heavy and light chains of pSh-CBD did not interact with benzo[a]pyren. Previously defined eleven pSh-CBD aa involved to benzo[a]pyren binding were confirmed by mutagenesis. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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