| Autor: |
Siddiqui, Ali A, Jones, Elizabeth, Andrade, Darren, Shah, Apeksha, Kowalski, Thomas E, Loren, David E, Chipitsyna, Galina, Arafat, Hwyda A |
| Předmět: |
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| Zdroj: |
Journal of Gastroenterology & Hepatology; Jun2014, Vol. 29 Issue 6, p1321-1327, 7p |
| Abstrakt: |
Background and Aim Osteopontin ( OPN) is a phosphoprotein that activates pathways that induce cancer cell survival and metastasis. Our aim was to examine the expression pattern of OPN splice variants a, b, and c in fine-needle aspirates and to determine their correlation with stage-adjusted pancreatic ductal adenocarcinoma ( PDA) survival. Methods Endoscopic ultrasound-guided fine-needle aspiration ( EUS- FNA) was performed in patients with solid pancreatic masses. The tissue was collected and analyzed for the expression of OPN isoforms by reverse transcription-polymerase chain reaction. Survival curves of stages and overexpression of OPN splice variants (a, b, c) were estimated according to the Kaplan- Meier and the log-rank test. Results EUS- FNA was performed in 46 patients with solid pancreatic lesions (40 PDA and 6 chronic pancreatitis). OPNa was highly expressed in 39/40 (98%), OPNb in 24/40 (60%), while OPNc was present in 10/40 (25%) of PDA samples. The median survival was lower in patients whose fine-needle aspiration ( FNA) samples expressed OPNb than those without (406 days vs 749 days, P = 0.049). There was no significant difference in survival in patients with OPNc. Cox proportional hazard model demonstrated that OPNb expression had a trend toward decrease overall survival ( P = 0.06), with these patients having a hazard of death three times higher than those without. OPNc was found to significantly correlate with metastatic disease ( P = 0.009) in PDA patients. Conclusions Our data show for the first time that in FNA samples, there is a strong association between OPNc and presence of metastasis in PDA, and OPNb and poor survival. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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