| Autor: |
Sun, Wei, Guan, Qun, Wen, Ji, Zhang, Qiyao, Yang, Weina, Zhang, Bin, Cui, Wei, Zou, Zhiquan, Yu, Yan |
| Předmět: |
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| Zdroj: |
Journal of Assisted Reproduction & Genetics; May2014, Vol. 31 Issue 5, p541-547, 7p |
| Abstrakt: |
Purpose: The aim of this study was to determine whether altered expression and distribution of calcium- and integrin-binding protein-1 (CIB1) is involved in the pathogenesis of patients with oligoasthenozoospermia. Methods: Sperm samples were obtained from 25 infertile Chinese men who had failed to achieve conception after a period of 1-2 y and had been referred to the Reproductive Laboratory of the second hospital affiliated to the Shandong University of Traditional Chinese Medicine. Participants were divided into two groups: oligoasthenozoospermia ( n = 13) and asthenozoospermia ( n = 12); as a third group, fertile men ( n = 19) were included as controls. The expression levels of mRNA and protein levels of CIB1 and cyclin-dependent kinase 1 (CDK1) were measured using qRT-PCR and western blotting. Results: mRNA and protein expression levels of CIB1 were decreased in the oligoasthenozoospermia patients. Interestingly mRNA and protein expression levels of CDK1 were increased in the oligoasthenozoospermia patients. Conclusion: The results of the present study indicate that that CIB1 may be involved in the pathogenesis of oligoasthenozoospermia by the CDK1 signaling pathway. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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