Autor: |
Clay, Dorina, Winkler, Christoph, Tasnádi, Gábor, Faber, Kurt |
Předmět: |
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Zdroj: |
Biotechnology Letters; Jun2014, Vol. 36 Issue 6, p1329-1333, 5p |
Abstrakt: |
The bioreduction and disproportionation of cyclohex-2-enone catalyzed by Old Yellow Enzyme 1 was investigated in presence of organic (co)solvents. Whereas the NADH-dependent bioreduction activity strongly decreased at elevated co-solvent concentrations due to the insolubility of the nicotinamide-cofactor, the NADH-free disproportionation was significantly improved in water-immiscible organic co-solvents at 90 % (v/v) with near-quantitative conversion. This positive effect was attributed to removal of the inhibiting co-product, phenol, from the enzyme's active site. The best co-solvents show high lipophilicity (logP) and a high potential to solubilize phenol (K). As a predictive parameter, the ratio of logP/K should be preferably ≥100. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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