| Autor: |
Yukinaga, Hiroko, Shionyu, Clara, Hirata, Eishu, Ui-Tei, Kumiko, Nagashima, Takeshi, Kondo, Shinji, Okada-Hatakeyama, Mariko, Naoki, Honda, Matsuda, Michiyuki |
| Předmět: |
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| Zdroj: |
Journal of Cell Science; 2014, Vol. 127 Issue 8, p1805-1815, 11p |
| Abstrakt: |
Phenotypic heterogeneity of cancer cells is caused not only by genetic and epigenetic alterations but also by stochastic variation of intracellular signaling molecules. Using cells that stably express Förster resonance energy transfer (FRET) biosensors, we show here a correlation between a temporal fluctuation in the activity of Rac1 and the invasive properties of C6 glioma cells. By using long-term time-lapse imaging, we found that Rac1 activity in C6 glioma cells fluctuated over a timescale that was substantially longer than that of the replication cycle. Because the relative level of Rac1 activity in each cell was unaffected by a suspension -- adhesion procedure, we were able to sort C6 glioma cells according to the levels of Rac1 activity, yielding Rac1[sup high] and Rac1[sup low] cells. The Rac1[sup high] cells invaded more efficiently than did Rac1[sup low] cells in a Matrigel invasion assay. We assessed the transcriptional profiles of Rac1[sup high] and Rac1[sup low] cells and performed gene ontology analysis. Among the 14 genes that were most associated with the term 'membrane' (membrane-related genes) in Rac1[sup high] cells, we identified four genes that were associated with glioma invasion and Rac1 activity by using siRNA knockdown experiments. Among the transcription factors upregulated in Rac1[sup high] cells, Egr2 was found to positively regulate expression of the four membrane-related invasion-associated genes. The identified signaling network might cause the fluctuations in Rac1 activity and the heterogeneity in the invasive capacity of glioma cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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