Sum Frequency Generation Vibrational Spectroscopy: A Sensitive Technique for the Study of Biological Molecules at Interfaces.

Autor: Boughton, Andrew P., Chen, Zhan
Zdroj: Surface Analysis & Techniques in Biology; 2014, p195-224, 30p
Abstrakt: The behavior of biological molecules in interfacial environments is critical to understanding a broad range of phenomena, from biocompatibility to the functions of membrane-associated peptides and proteins. Sum frequency generation (SFG) vibrational spectroscopy is a nonlinear optical vibrational spectroscopic technique with an excellent sensitivity to interfacial molecules and molecular ordering and is well suited to probing biomolecules in a native interfacial environment. Using this technique, one can obtain unique information on the biomolecular orientation and conformation at interfaces. SFG also provides additional measurements that are complementary to other vibrational studies; more complicated protein structures and orientation distributions may be studied in greater detail when SFG is combined with other vibrational techniques. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index