Immunogenicity of recombinant human bocavirus-1,2 VP2 gene virus-like particles in mice.

Autor: Deng, Zhong‐Hua, Hao, Ye‐Xia, Yao, Li‐Hong, Xie, Zhi‐Ping, Gao, Han‐Chun, Xie, Le‐Yun, Zhong, Li‐li, Zhang, Bing, Cao, You‐De, Duan, Zhao‐Jun
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Zdroj: Immunology; May2014, Vol. 142 Issue 1, p58-66, 9p
Abstrakt: Human bocavirus ( HBo V), a recently identified pathogen with a worldwide distribution is closely related to paediatric acute respiratory infection and gastroenteritis. The present study was performed to evaluate the immunogenicity of HBo V1 and HBo V2 virus-like particles ( VLPs) as vaccine candidates in mice. Both HBo V1 and HBo V2 VLPs were expressed in the bacmid virus- SF9 cell system. Mice were inoculated three times at 3-week intervals with HBo V VLPs at one dose intramuscular (i.m.) or intradermal (i.d.) with or without the addition of the alum adjuvant. ELISA was used to detected antibody, and ELISPOT was used to test cellular immune responses. HBo V-specific Ig G antibodies were induced and alum adjuvant improved the antibody titres and avidity, while the inoculation pathway had no influence. T helper type 1/ type 2 immune responses were balanced induced by HBo V1 VLPs but not HBo V2 VLPs. Serum Ig G antibody cross-reactivity rates of the two subtypes were similar, but cross-reactions of HBo V1 immunization groups were higher. The single i.m. group had more interferon- γ-secreting splenocytes. These data indicate that HBo V VP2 VLPs have good immunogenicity with induction of strong humoral and cellular immune responses, and they may be potential candidate vaccines for HBo V infection. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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