| Abstrakt: |
Aims The relationship between hydroxyl radical (· OH) and oxidatively modified macromolecule formations was examined in tissues from young and aged mice. Methods To determine the · OH generation in tissues in vivo using the hydroxylation trapping reaction of · OH into salicylic acid ( SA), analytical conditions for dihydroxybenzoic acid ( DHBA) and SA determination, and optimum dosages of SA for administration and time-points of tissue sampling were determined. 2, 3- DHBA levels in tissues from young mice and age-related changes were determined with the oxidatively modified macromolecules. Results 2, 3- DHBA, a hydroxylation compound of SA, is considered to be suitable for determination of · OH levels in tissues. Tissue levels of 2, 3- DHBA expressed as a molar ratio to SA, was comparable among tissues, and was in accordance with 8-oxo-2′-deoxyguanosine (8-oxod G) and carbonylated proteins. In the aging process, 2, 3- DHBA levels in the brain and heart increased in the biphasic pattern in accordance with the 8-oxodG and thiobarbituric acid reactive substances ( TBARS) levels, whereas levels of carbonylated proteins were not changed with age. Conclusions An in vivo method for · OH measurement using hydroxylation of SA was optimized. However, as a limitation, 2, 3- DHBA, as well as other oxidative stress markers, could be affected by various in vivo factors. The accordance was seen among 2, 3- DHBA, 8-oxod G and carbonylated protein levels in tissues from young mice. The tissue levels of 2, 3- DHBA increased in accordance with the 8-oxod G and TBARS during the aging process. Geriatr Gerontol Int 2014; 14: 498-507. [ABSTRACT FROM AUTHOR] |
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