| Autor: |
Sripakdeevong, Parin, Cevec, Mirko, Chang, Andrew T, Erat, Michèle C, Ziegeler, Melanie, Zhao, Qin, Fox, George E, Gao, Xiaolian, Kennedy, Scott D, Kierzek, Ryszard, Nikonowicz, Edward P, Schwalbe, Harald, Sigel, Roland K O, Turner, Douglas H, Das, Rhiju |
| Předmět: |
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| Zdroj: |
Nature Methods; Apr2014, Vol. 11 Issue 4, p413-416, 4p |
| Abstrakt: |
Structured noncoding RNAs underlie fundamental cellular processes, but determining their three-dimensional structures remains challenging. We demonstrate that integrating 1H NMR chemical shift data with Rosetta de novo modeling can be used to consistently determine high-resolution RNA structures. On a benchmark set of 23 noncanonical RNA motifs, including 11 'blind' targets, chemical-shift Rosetta for RNA (CS-Rosetta-RNA) recovered experimental structures with high accuracy (0.6-2.0 Å all-heavy-atom r.m.s. deviation) in 18 cases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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