| Autor: |
Zainal Ariffin, Shahrul Hisham, Kermani, Shabnam, Zainol Abidin, Intan Zarina, Abdul Wahab, Rohaya Megat, Yamamoto, Zulham, Senafi, Sahidan, Ariffin, Zaidah Zainal, Razak, Mohamad Abdul |
| Předmět: |
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| Zdroj: |
Stem Cells International; 2013, p1-10, 10p |
| Abstrakt: |
Dental pulp tissue contains dental pulp stem cells (DPSCs). Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells) are capable of differentiating intomultilineage cells including neuron-like cells. The aimof this study was to examine the capability of DPSCs to differentiate into neuron-like cells without using any reagents or growth factors. DPSCs were isolated from teeth extracted from6- to 8-week-old mice and maintained in complete medium. The cells fromthe fourth passage were induced to differentiate by culturing in mediumwithout serumor growth factors.RT-PCRmolecular analysis showed characteristics of Cd146+, Cd166+, and Cd31- in DPSCs, indicating that these cells are mesenchymal stem cells rather than hematopoietic stem cells. After 5 days of neuronal differentiation, the cells showed neuron-like morphological changes and expressedMAP2 protein. The activation of Nestin was observed at low level prior to differentiation and increased after 5 days of culture in differentiation medium, where as Tub3 was activated only after 5 days of neuronal differentiation. The proliferation of the differentiated cells decreased in comparison to that of the control cells. Dental pulp stem cells are induced to differentiate into neuron-like cells when cultured in serum- and growth factor-free medium. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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