Purification of an MCM-containing complex as component of the DNA replication licensing system.

Autor: Chong, James P.J., Mahbubani, Hiro M.
Předmět:
Zdroj: Nature; 6/1/1995, Vol. 375 Issue 6530, p418, 4p, 5 Black and White Photographs, 8 Graphs
Abstrakt: Describes the assay for the purification of replication licensing factor from Xenopus eggs. Resolution of RLF-M and RLF-B components; Resemblance of RLF-M with yeast MCM3 protein; Association of Xenopus MCM3 with chromatin in the G1 phase of cell cycle; Removal of Xenopus MCM3 during replication; Explanation for the replication of DNA once in every cell cycle.
Databáze: Complementary Index