| Autor: |
Sparrow, Rosemary L., Sran, Amrita, Healey, Geraldine, Veale, Margaret F., Norris, Philip J. |
| Předmět: |
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| Zdroj: |
Transfusion; Mar2014, Vol. 54 Issue 3, p560-568, 9p |
| Abstrakt: |
Background Saline-adenine-glucose-mannitol ( SAGM) and a variant solution, AS-1, have been used for more than 30 years to preserve red blood cells ( RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired study of SAGM RBCs and AS-1 RBCs was conducted to identify membrane changes, including microparticle ( MP) quantitation and in vitro RBC-endothelial cell ( EC) interaction. Study Design and Methods Two whole blood packs were pooled and split and RBCs were prepared (n = 6 pairs). One pack was suspended in SAGM and one in AS-1. Samples were collected during 42 days of refrigerated storage. RBC shape and size and glycophorin A ( GPA)+ and phosphatidylserine ( PS)+ MPs were measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro flow perfusion assay. Routine variables ( pH, hemolysis) were also measured. Results Compared to SAGM RBCs, AS-1 RBCs had lower hemolysis (p < 0.04), lower GPA+ MPs (p < 0.03), and lower PS+ MPs (p < 0.03) from Day 14 onward. AS-1 RBCs had higher (p < 0.02) side scatter from Day 28 onward compared to SAGM RBCs. SAGM RBCs were more adherent to ECs on Day 28 of storage compared to AS-1 RBCs (p = 0.04), but reversed on Day 42 (p = 0.02). Conclusion SAGM RBCs lose more membrane during storage. SAGM RBCs had increased adherence to ECs on Day 28 of storage, while AS-1 RBCs were more adherent on Day 42. The effect of these differences on the function and survival of SAGM RBCs and AS-1 RBCs after transfusion remains to be determined. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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