| Autor: |
Kaur, Harshaminder, Jindal, Sachin, Manjunath, S. M., Garg, Hemant |
| Předmět: |
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| Zdroj: |
Indian Journal of Dental Sciences; Oct2013, Vol. 5 Issue 4, p19-21, 3p |
| Abstrakt: |
Background: Dental calculus is a form of calcification in the oral cavity, assembled through interaction with dental plaque which is a community of microorganisms found on the tooth surface. Studies have stated that once the plaque matures to form dental calculus, bacteria residing within channels and lacunae are killed, but still the inflammatory process is going on within the soft tissues. Therefore, the present study was conducted to identify thethe presence or absence of viable bacteria within dental calculus. Materials and Methods: Supragingival and subgingival calculus were harvested from 30 subjects having the clinical evidence of chronic inflammatory periodontal disease. Harvested calculus samples were placed immediately in sterile saline and crushed aseptically between two sterile slides and examined using grams stain, fluorescent microsopy using acridine orange and dark field microscopy. Results: Gram staining revealed the presence of gram -ve bacteria in the form rod shaped bacilli, cocci and filamentous bacteria either singly or in groups within the sample. Acridine orange fluorescent stain showed the presence of viable bacteria which formed 40% of all the microorganisms and dark field microscopic examination revealed the presence of motile bacteria within the sample. Conclusion: From the result it appears that bacteria are present within dental calculus, specifically within the channels and lacunae. Thus, calculus may serve as a reservoir of viable micro organisms and play a crucial role in the etiology and recurrence of oral infections even after treatment. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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