| Autor: |
Leung, Lawrence W., Contreras, Ruben G., FLores-Maldonado, Catalina, Cereijido, Marcelino, Rodriguez-Boulan, Enrique |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Cell Physiology; Apr2003, Vol. 284 Issue 4, pC1021, 10p, 12 Color Photographs, 10 Black and White Photographs, 1 Diagram, 6 Graphs |
| Abstrakt: |
Madin-Darby canine kidney (MDCK) I and Fisher rat thyroid (FRT) cells exhibit transepithelial electrical resistance (TER) values in excess of 5,000 Ω·cm². When these cells were incubated in the presence of various inhibitors of sphingolipid biosynthesis, a >5-fold reduction of TER was observed without changes in the gate function for uncharged solutes or the fence function for apically applied fluorescent lipids. The localization of ZO-1 and occludin was not altered between control and inhibitortreated cells, indicating that the tight junction was still intact. Furthermore, the complexity of tight junction strands, analyzed by freeze-fracture microscopy, was not reduced. Once the inhibitor was removed and the cells were allowed to synthesize sphingolipids, a gradual recovery of the TER was observed. Interestingly, these inhibitors did not attenuate the TER of MDCK II cells, a cell line that typically exhibits values below 800 Ω·cm². These results suggest that glycosphingolipids play a role in regulating the electrical properties of epithelial cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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