| Autor: |
Abedi, Reyhaneh, Eimani, Hussein, Pashaee Rad, Shahrokh, Eftekhari Yazdi, Popak, Shahverdi, Abdol Hossein, Mokhber Maleki, Elham |
| Předmět: |
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| Zdroj: |
Reproductive Medicine & Biology; Jan2014, Vol. 13 Issue 1, p29-35, 7p |
| Abstrakt: |
Purpose: Allopurinol and FSH injection are applied to reduce ischemia-reperfusion injury and to increase survival rate for ovarian follicles after ovarian heterotopic autotransplantation in mice. Methods: Ovarian tissues from 6-week-old mice were grafted into back muscle then collected after 3 weeks. A total of five groups were included in this experiment as follows: control group ( n = 5), sham-operated group ( n = 5), allopurinol treatment group (AP) ( n = 5), follicle stimulating hormone (FSH) treatment group ( n = 5), as well as, allopurinol and FSH treatment group (APF) ( n = 5). We investigated survival, number and development of follicles, vaginal cytology along with plasma malondialdehyde (MDA) concentration in grafted ovary. Results: Total follicles count significantly increased in APF group compared with other treatment groups ( p < 0.05). MDA concentration significantly decreased in AP group and APF treatment group compared with sham-operated group. In AP group, vaginal smears showed presence of cornified epithelial cells three-five day after surgery. Conclusions: We demonstrated that allopurinol, as a XO inhibitor, plays an important role in order to decrease ischemia injury and to increase survival rate for follicles. Also, FSH, as a folliculogenesis and angiogenesis factor, increases development of follicles. It seems that allopurinol can cause re-establishing hypothalamus-pituitary axis and finally can restore estrous cycle earlier than for the sham operated group, so it explains the increasing survival rate for follicles. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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