Dysfunction of alveolar macrophages after cardiac surgery: Predisposing factor for the development of postoperative pneumonia? -- an observational study.

Autor: Chalk, Katharina, Meisel, Christian, Spies, Claudia, Volk, Thomas, Thuenemann, Karin, Linneweber, Jörg, Wernecke, Klaus-Dieter, Sander, Michael
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Zdroj: Critical Care; 2013, Vol. 17 Issue 6, p1-18, 18p, 4 Charts, 3 Graphs
Abstrakt: Introduction Patients undergoing cardiac surgery have an increased risk of postoperative pneumonia. Pulmonary immune dysfunction might be a contributing factor. We therefore determined changes of the surface molecules on alveolar macrophages (AMs). To characterize modulation in patients with pneumonia we correlated these changes to the development of postoperative pneumonia. Methods After ethical approval and written informed consent, 33 patients undergoing elective coronary bypass grafting surgery were included in this observational study. Peripheral blood cells and alveolar lavage fluid were collected directly after induction of anesthesia and two hours after separation from cardiopulmonary bypass (CPB). Human leukocyte antigen-DR (HLA-DR) and toll-like receptors (TLR) 2/4 expression on monocytes and AM were assessed by flow cytometry. A total of three patients developed postoperative pneumonia; determined according to the criteria of the Center of Disease Control. Statistical analysis was performed with the Mann-Whitney-U test and Wilcoxon test. Results We found significant changes of phenotypic and functional immune markers on AMs after cardiac surgery. HLA-DR expression on peripheral blood monocytes and AMs was significantly reduced compared to baseline in all patients (each approximately 30 %). After surgery patients who developed postoperative pneumonia revealed a trend of stronger reduction of HLA-DR expression (83.7 % versus 27.1 %) and TLR4 expression on AMs (46.1 % versus 9.9 %) compared to patients without pneumonia. Already before surgery, the baseline of TLR2 expression on AM was significantly lower (27.7 %) in patients who developed postoperative pneumonia. Conclusions As far as we know this is the first study that shows an early impairment of lung cellular immune response after cardiac surgery. These findings can help to understand the role of cell- mediated immunosuppression and its association to the development of postoperative pneumonia. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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