Molecular investigation of zoonotic genotypes of Giardia intestinalis isolates in humans, dogs and cats, sheep, goats and cattle in Araçatuba (São Paulo State, Brazil) by the analysis of β -giardin gene fragments.

Autor: de Godoy, Elenir Alves Macedo, Santos Junior, Juares Elias, Teresa Belloto, Marcus Vinícius, de Moraes, Marcus Vinícius Proença, Cassiano, Gustavo Capatti, Caseca Volotão, Aline Cardoso, Rui Luvizotto, Maria Cecília, Aparecida Carareto, Claudia Márcia, de Moraes Silva, Mônica Cristina, Dantas Machado, Ricardo Luiz
Předmět:
Zdroj: Microbiology Research; 2013, Vol. 4 Issue 1, p26-31, 6p
Abstrakt: In the period from July 2009 to October 2010, fecal samples from 61 animals and 154 humans from the municipality of Araçatuba (São Paulo State, Brazil) were studied. Fecal samples from animals were collected in the Municipal Animal Shelter and the Veterinary Hospital of the Universidade Estadual Paulista. Human fecal specimens were collected in playschools in the outskirts of the city by the private network of clinical analysis laboratories of the municipal. Diagnosis was done by optical microscopy using the Faust and Hoffmann, Pons and Janer techniques. The genotypes of Giardia intestinalis were characterized by PCR-RFLP and confirmed by sequencing the ß-giardin gene. Human specimens were positive in 25.3% (39/154) of the cases with 26.8% (36/134) of the specimens from children and 15% (3/20) from adults being positive. The frequency of G. intestinalis among the animals was 23.0% (14/61). A total of 32 isolates of G. intestinalis obtained from human feces and six from dogs and cats were characteristic of the A genotype (AI and AII/AIII). The results of this study in respect to frequency of giardiasis are similar to reported in most studies in Brazil. The prevalence observed in animal populations conforms to worldwide infection rates. G. intestinalis genotypes considered zoonotic were detected in both pets and humans from the city of Araçatuba, suggesting a possible zoonotic transmission of the parasite in the northwestern region of São Paulo State. The absence of these genotypes in farm animals may imply that they are not involved in the chain of transmission to humans in this region. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index