| Autor: |
Musa Tibin, El Mubarak, Naseer Al-Shorgani, Najeeb Kaid, Abuelhassan, Nawal Noureldaim, Hamid, Aidil Abdul, Kalil, Mohd Sahaid, Wan Mohtar Wan Yusoff |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; Dec2013, Vol. 1571 Issue 1, p184-190, 7p, 4 Charts, 3 Graphs |
| Abstrakt: |
The cellulase production using sorghum straw as substrate by fungal culture of Aspergillus terreus SUK-1 was investigated in solid substrate fermentation (SSF). The optimum CMCase was achieved by testing most effective fermentation parameters which were: incubation temperature, pH and moisture content using Response Surface Methodology (RSM) based on Central Composite Design (CCD).The carboxymethyl cellulase activity (CMCase) was measured as the defining factor. The results were analysed by analysis of variance (ANOVA) and the regression quadratic model was obtained. The model was found to be significant (p<0.05) and the effect of temperature (25-40°C) and pH (4-7) was found to be not significant on CMCase activity whereas the moisture content was significant in the SSF conditions employed. The high yield of predicted CMCase activity (0.2 U/ml) was obtained under the optimized conditions (temperature 40C, pH 5.4 and moisture content of 80%). The model was validated by applying the optimized conditions and it was found that the model was valid. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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