| Autor: |
Hoxie, Neil J., Vergeront, James M., Pfister, John R., Hoffman, Gary L., Markwardt-Elmer, Pamela A., Davis, Jeffrey P. |
| Předmět: |
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| Zdroj: |
American Journal of Public Health; Oct92, Vol. 82 Issue 10, p1370-1373, 4p, 1 Chart, 1 Graph |
| Abstrakt: |
Objectives. Nationwide, human immunodeficiency virus type 1 (HIV-1) seroprevalence surveys using dried neonatal blood specimens are critical to estimating HIV-1 sero-prevalence among childbearing women. However, the noninclusion of blood specimens deemed "quantity not sufficient" (QNS) for HIV-1 antibody testing potentially introduces bias. In Wisconsin beginning in 1990, we modified the survey protocol to reduce QNS rates and assess bias introduced by QNS specimens. methods. The HIV-1 antibody assay was modified to use four 1/8 -in blood spots when a single 1/4-in blood spot could not be obtained. Both methods obtain identical blood volumes for testing. Results. During a 27-month period, 7396 (4.8%) of 154 683 specimens were deemed QNS using 1/4-in blood spots. Of these, 6590 (89%) were of sufficient quantity to be tested using four 1/8-in blood spots; 6 (0.09%) specimens tested with 1/8-in blood spots were HIV-1 Western blot assay positive compared with 44 (0.03%) of 147 287 1/4-in specimens (odds ratio = 3.0; 95% confidence interval = 1.2, 7.4). Conclusions. Because noninclusion of QNS specimens potentially introduces bias, incorporating the results of HIV-1 antibody testing of QNS specimens using four 1/8-in blood spots can improve the accuracy of HIV-1 seroprevalence estimates in these serologic surveys. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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