| Autor: |
Nyi Sim, Adrian Chong, Chien Tei Too, Min Zin Oo, Junyun Lai, Eio, Michelle Yating, Zhenying Song, Srinivasan, Nalini, Diane Ai Lin Tan, Shyue Wei Pang, Shu Uin Gan, Kok Onn Lee, Seng Loh, Thomas Kwok, Jianzhu Chen, Soh Ha Chan, MacAry, Paul Anthony |
| Předmět: |
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| Zdroj: |
Scientific Reports; 11/22/2013, p1-7, 7p |
| Abstrakt: |
Epstein-Barr virus (EBV) is a gamma herpesvirus that causes a life-long latent infection in human hosts. The latent gene products LMP1, LMP2A and EBNA1 are expressed by EBV-associated tumors and peptide epitopes derived from these can be targeted by CD8 Cytotoxic T-Lymphocyte (CTL) lines. Whilst CTL-based methodologies can be utilized to infer the presence of specific latent epitopes, they do not allow a direct visualization or quantitation of these epitopes. Here, we describe the characterization of three TCR-like monoclonal antibodies (mAbs) targeting the latent epitopes LMP1125-133, LMP2A426-434 or EBNA1562-570 in association with HLA-A0201. These are employed to map the expression hierarchy of endogenously generated EBV epitopes. The dominance of EBNA1562-570 in association with HLA-A0201 was consistently observed in cell lines and EBV-associated tumor biopsies. These data highlight the discordance between MHC-epitope density and frequencies of associated CTL with implications for cell-based immunotherapies and/or vaccines for EBV-associated disease. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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