| Autor: |
Medina-Canales, Ma.G., Rodríguez-Tovar, A.V., Manzanilla-López, R.H., Zúñiga, G., Tovar-Soto, A. |
| Předmět: |
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| Zdroj: |
Biocontrol Science & Technology; Jan2014, Vol. 24 Issue 1, p1-21, 21p |
| Abstrakt: |
New Mexican isolates of the nematophagous fungusPochonia chlamydosporiawere obtained from nematode infested fields in the vegetable growing area of Tepeaca Valley, Puebla State, Mexico. Based on macro and microscopic morphology, seven ‘putative’P. chlamydosporiaisolates were selected and the DNA extracted for polymerase chain reaction (PCR). Three new isolates ofP. chlamydosporiawere identified: Pcp2, Pcp21 and Pcp31. The amplification reaction of the internal transcribed spacer (ITS) region revealed a 650 bp amplicon which was used in a maximum likelihood phylogenetic inference analysis. Three groups were recovered in the tree topology, supported bya> 90% bootstrap value. Nucleotide identity values were > 83.6% between the test sequences and the reference sequence. In addition, using specific primers for two existing varieties ofP. chlamydosporia, restriction fragment length polymorphism on the ITS products in conjunction with the phylogenetic inferences and the molecular test for detection ofP.chlamydosporia vcp1gene, it was found that all three isolates belong to a new variety which we have namedP. chlamydosporiavar.mexicana. We compared the chlamydospore production rate, rhizosphere colonisation and egg parasitism percentages of the three native isolates inMeloidogynespp. with a reference isolate (Pc10). Native isolates produced > 1×106chlamydospores/50 g of substrate (of which more than 80% were viable), colonised > 80% of the rhizosphere, and parasitised > 60% ofMeloidogyne incognitaandMeloidogyne arenariaeggs.Meloidogyne haplaegg parasitism was < 60%. Isolates Pcp2 and Pcp21 were identified as potential biological control agents ofMeloidogynespp. to be tested further in greenhouse and field tests. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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