| Autor: |
Stein, Torsten, Heinzmann, Stefan, Kiesau, Peter, Himmel, Bettina, Entian, Karl-Dieter |
| Předmět: |
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| Zdroj: |
Molecular Microbiology; Mar2003, Vol. 47 Issue 6, p1627-1636, 10p |
| Abstrakt: |
Summary The subtilin gene cluster (spa ) of Bacillus subtilis ATCC 6633 is organized in transcriptional units spaBTC , spaS , spaIFEG and spaRK . Specific binding of the response regulator protein SpaR to spaB , spaS and spaI DNA promoter fragments was shown by means of electromobility shift assays. A repeated pentanucleotide sequence spaced by six nucleotides was identified as SpaR binding motif (spa-box ). Saturating mutational analysis of the spa-box by single- and multiple-base-pair substitutions revealed the consensus motif (A/T)TGAT for optimal SpaR binding with the second, third and fifth position being absolutely conservative. Variations in the spacer size between the two pentanucleotide repeats revealed a strong conservation of their relative location. Only DNA with a proximal arrangement of two pentanucleotide repeats showed affinity to SpaR. A 2:1 stoichiometry between SpaR and DNA was obtained by optical biosensor analyses, which corresponds to the binding of two SpaR proteins per spa-box . [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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