| Autor: |
Hovis, Kelley M., Mojica, Sergio, McDermott, Jason E., Pedersen, Laura, Simhi, Chana, Rank, Roger G., Myers, Garry S.A., Ravel, Jacques, Hsia, Ru-ching, Bavoil, Patrik M. |
| Předmět: |
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| Zdroj: |
Pathogens & Disease; Dec2013, Vol. 69 Issue 3, p213-222, 10p |
| Abstrakt: |
Among chlamydial virulence factors are the type III secretion ( T3 S) system and its effectors. T3 S effectors target host proteins to benefit the infecting chlamydiae. The assortment of effectors, each with a unique function, varies between species. This variation likely contributes to differences in host specificity and disease severity. A dozen effectors of Chlamydia trachomatis have been identified; however, estimates suggest that more exist. A T3 S prediction algorithm, SVM-based Identification and Evaluation of Virulence Effectors ( SIEVE), along with a Yersinia surrogate secretion system helped to identify a new T3 S substrate, CT082, which rather than functioning as an effector associates with the chlamydial envelope after secretion. SIEVE was modified to improve/expand effector predictions to include all sequenced genomes. Additional adjustments were made to the existing surrogate system whereby the N terminus of putative effectors was fused to a known effector lacking its own N terminus and was tested for secretion. Expansion of effector predictions by c SIEVE and modification of the surrogate system have also assisted in identifying a new T3 S substrate from C. psittaci. The expanded predictions along with modifications to improve the surrogate secretion system have enhanced our ability to identify novel species-specific effectors, which upon characterization should provide insight into the unique pathogenic properties of each species. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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