| Autor: |
Shweta, S., Kannan, K., Madhavan, S., Adhithya, R., Paranidharan, V., Velazhahan, R. |
| Předmět: |
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| Zdroj: |
Archives of Phytopathology & Plant Protection; Nov2013, Vol. 46 Issue 18, p2174-2181, 8p |
| Abstrakt: |
Aflatoxin contamination is a major problem in maize, groundnut, chillies, cotton and tree nuts. These aflatoxins are low molecular weight toxic and carcinogenic secondary metabolites produced byAspergillus flavus,A. parasiticusandA. nomius. In the present study, a total of 11 isolates ofA. flavusisolated from groundnut, maize and chilli collected from different locations of Tamil Nadu, India were tested for their ability to produce aflatoxin B1 (AFB1)in vitroby indirect competitive enzyme-linked immunosorbent assay. The results show that the isolates vary in their level of toxin production. The amount of AFB1 produced by the toxigenic isolates ofA. flavusranged from 6.6 to 108.1 ng ml−1. Among the various isolates ofA. flavus, the isolate VKR produced the highest amount (108.1 ng ml−1) of AFB1. The isolates viz. CBE1, CBE2, BSR1, BSR3 and BSR4 were found to be non-toxigenic. The genetic variability among these isolates was assessed by Random amplified polymorphic DNA (RAPD) analysis. DNA fragments of between 0.15 and 3.0 kb were obtained using 13 random primers, and each isolate differed in the size and number of PCR products indicating considerable polymorphism. Cluster analysis using Unweighted Pair Group Method with Arithmetic Mean clearly separated the isolates into four main clusters confirming the genetic diversity among the isolates ofA. flavus. Both toxigenic and non-toxigenic isolates were intermingled in these four groups, indicating that no relationship exists between RAPD profile and the production of aflatoxin byA. flavus. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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