| Autor: |
Marazza, José A., Nazareno, Mónica A., Savoy de Giori, Graciela, Garro, Marisa S. |
| Předmět: |
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| Zdroj: |
International Journal of Food Science & Technology; Dec2013, Vol. 48 Issue 12, p2480-2489, 10p |
| Abstrakt: |
Bioconversion of isoflavone glucosides and antioxidant activity by probiotic strain ( Bifidobacterium longum) during soymilk fermentation was investigated, as well as partial characterisation of the produced enzyme β-glucosidase. The enzyme has higher affinity for genistin than for other substrates assayed. Maximum activity occurred at 42 °C and at pH 6.0; keeping 70-80% of activity for 60 days stored at low temperatures. Bifidobacterium longum grew well in soymilk (8.26 log CFU mL−1 and pH of 3.9 at 24 h) and were produced in good quantities of organic acids. High hydrolysis degree of isoflavone glucosides (81.2%) was observed at 24 h. Enhancements in bioactivity were assessed in fermented soymilk by monitoring the radical-scavenging activity, antioxidant activity and DNA protective action. The use of probiotic Bifidobacterium strain as β-glucosidase producer increased bioactive isoflavone content and demonstrated that this enzyme plays a key role in the bioavailability of soymilk isoflavones, reducing the bioconversion time compared to other studies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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