| Abstrakt: |
Gingival crevicular fluid (GCF) has emerged as a useful, non-invasive method for assessing tissue responses. The intra-cytoplasmic enzyme Aspartate aminotransferase (AST) is released into the extra-cellular environment upon cell death. The aim of the present study was to evaluate the effect of orthodontic tooth movement on AST activity in GCF. A total of 7 female patients requiring orthodontic treatment with Pre-adjusted Edgewise appliance (Roth prescription,0 .022'' x 0.028'' slot) and first premolar extractions were included in the study. The GCF was collected from mesial & distal gingival margins of maxillary right canine (Test tooth) & left canine (Control tooth) at the following intervals- before placement of appliance, at the initiation of canine retraction and at 1st, 7th, 14th, and 28th day after force application. For comparisons among and within the groups - Paired t-test, unpaired t-test and ANOVA were used in the study. Plaque Index (PI) and Gingival Index (GI) scores for individual patients were obtained at different time intervals. AST activity was assessed for each subject at four sites: test mesial (TM), test distal (TD), control mesial (CM) and control distal (CD) at different time intervals. The GCF AST activity values were found to be higher on the mesial and distal surfaces of the test tooth (TM and TD) as compared to the control tooth (CM and CD) on day 0 while the enzyme activity was more at the compression surface than at the tension surface on the 1st, 7th & 14th days. Thus, it was concluded within the limitations of the present study, GCF AST activity can be proposed as a sensitive biomarker of the periodontal metabolic changes during orthodontic tooth movement. [ABSTRACT FROM AUTHOR] |
