Effect of chronic Li+ treatment on free intracellular Mg 2+ in human neuroblastoma SH-SY5Y cells.

Autor: Abukhdeir, Abde M, Layden, Brian T, Minadeo, Nicole, Bryant, Fred B, Stubbs, Evan B., De Freitas, Duarte Mota
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Zdroj: Bipolar Disorders; Feb2003, Vol. 5 Issue 1, p6-13, 8p
Abstrakt: Objectives: Previous findings have demonstrated Li+ /Mg 2+ competition at therapeutic intracellular Li + levels after acute Li + treatment in human neuroblastoma SH-SY5Y cells. In the current study, we examined whether Li + /Mg 2+ competition exists at therapeutically relevant extra- and intracellular [Li + ] after chronic Li + loading times. Methods: In human neuroblastoma cells, intracellular free Mg2+ was determined by fluorescence spectroscopy with the fluorophore furaptra. Intracellular Li + and Mg 2+ were measured by atomic absorption spectrophotometry. Results: After loading of the neuroblastoma cells with 1–2 mM extracellular Li+ for 24–72 h, the observed, increased intracellular free [Mg 2+ ] levels were significantly higher (p < 0.03) than those in matched Li + free cells, and intracellular [Li + ] was found to be at therapeutic intracellular levels (0.7–1.5 mM). Conclusions: The results demonstrate that Li+ /Mg 2+ competition exists after chronic treatment with Li + at therapeutically relevant intracellular Li + levels in neuroblastoma cells. We found differences between acute and chronic Li + treatment effects on the extent of Li + /Mg 2+ competition. Possible reasons for these differences are discussed. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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