A Toll-like receptor 2 ligand, Pam3 CSK4, augments interferon-γ-induced nitric oxide production via a physical association between MyD88 and interferon-γ receptor in vascular endothelial cells.

Autor: Tsolmongyn, Bilegtsaikhan, Koide, Naoki, Jambalganiin, Ulziisaikhan, Odkhuu, Erdenezaya, Naiki, Yoshikazu, Komatsu, Takayuki, Yoshida, Tomoaki, Yokochi, Takashi
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Zdroj: Immunology; Nov2013, Vol. 140 Issue 3, p352-361, 10p
Abstrakt: The effect of Pam3CSK4, a Toll-like receptor 2 ( TLR2) ligand, on interferon-γ ( IFN-γ) -induced nitric oxide ( NO) production in mouse vascular endothelial END-D cells was studied. Pre-treatment or post-treatment with Pam3CSK4 augmented IFN-γ-induced NO production via enhanced expression of an inducible NO synthase ( iNOS) protein and mRNA. Pam3CSK4 augmented phosphorylation of Janus kinase 1 and 2, followed by enhanced phosphorylation of signal transducer and activator of transcription 1 ( STAT1) at tyrosine 701. Subsequently, the enhanced STAT1 activation augmented IFN-γ-induced IFN-regulatory factor 1 expression leading to the iNOS expression. Pam3CSK4 also induced the activation of p38 and subsequent phosphorylation of STAT1 at serine 727. A pharmacological p38 inhibitor abolished the augmentation of IFN-γ-induced NO production by Pam3CSK4. Surprisingly, Pam3CSK4 enhanced a physical association of MyD88 and IFN-γ receptor. Together, these findings suggest that Pam3CSK4 up-regulates IFN-γ signalling in vascular endothelial cells via the physical association between MyD88 and IFN-γ receptor α, and p38-dependent serine 727 STAT1 phosphorylation. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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