| Autor: |
Sprenger, Wander W., Dijkstra, Annereinou, Zwart, Gabriel J.M., van Agterveld, Miranda P., van Noort, Paul C.M., Parsons, John R. |
| Předmět: |
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| Zdroj: |
FEMS Microbiology Ecology; Feb2003, Vol. 43 Issue 1, p45, 9p |
| Abstrakt: |
The effect of competition for macroelements with bacteria from ditch water on the parathion-hydrolyzing Flavobacterium sp. ATCC 27551 (FB) was investigated within mixed continuous cultures under carbon-, nitrate- or phosphate-limited conditions. The high initial rate of parathion hydrolysis decreased rapidly in all cultures due to the loss of strain FB. Addition of 2-isopropyl-6-methyl-4-pyrimidinol (a selective source of carbon, nitrogen and energy for FB) to one nitrate- and carbon-limited chemostat caused a 20-fold increase in parathion-hydrolyzing activity compared to unamended control cultures and retention of FB. The presence of the parathion hydrolase-encoding gene could be demonstrated by a newly developed PCR detection method in all FB cultures during most of the cultivation period. These results suggest that competition effects cause the pesticide-degrading capacity of microbial communities depending on their frequency of exposure to the pesticide compounds. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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