| Autor: |
Qi Liu, Qingqing Yan, Yin Liu, Fang Hong, Zhenfei Sun, Leilei Shi, Ying Huang, Yuda Fang |
| Předmět: |
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| Zdroj: |
Plant Physiology; Sep2013, Vol. 163 Issue 1, p108-117, 10p |
| Abstrakt: |
MicroRNAs (miRNAs) are a class of small regulatory RNAs that are found in almost all of the eukaryotes. Arabidopsis (Arabidopsis thaliana) miRNAs are processed from primary miRNAs (pri-miRNAs), mainly by the ribonudease III-like enzyme DICER-LIKE1 (DCL1) and its specific partner, HYPONASTIC LEAVES1 (HYL1), a double-strand RNA-binding protein, both of which contain two doublestrand RNA-binding domains (dsRBDs). These dsRBDs are essential for miRNA processing, but the tractions of them are not dear. Here, we report that the two dsRBDs of DCL1 (DCL1-DID2), and to some extent the second dsRBD (DCL1-D2), complement the hyll mutant, but not the first dsRBD of DCL1 (DCL1-D1). DCL1-D1 is diffusely distributed throughout the nucleoplasm, whereas DCL1-D2 and DCL1-DID2 concentrate in nuclear dicing bodies in which DCL1 and HYL1 colocalize. We show further that protein-protein interaction is mainly mediated by DCL1-D2, while DCL1-D1 plays a major role in binding of pri-miRNAs. These results suggest parallel roles between C-terminal dsRBDs of DCL1 and N-terminal dsRBDs of HYL1 and support a model in which Arabidopsis pri-miRNAs are recruited to dicing bodies through functionally divergent dsRBDs of microprocessor for accurate processing of plant pri-miRNAs. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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