| Autor: |
Poitry, Serge, van Bever, Laurianne, Coppex, Fabrice, Roatti, Angela, Baertschi, Alex J. |
| Předmět: |
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| Zdroj: |
Cardiovascular Research; Feb2003, Vol. 57 Issue 2, p468, 9p |
| Abstrakt: |
Objective: The aim is to compare the activation of ATP-sensitive potassium channels (KATP channels) in intact and metabolically impaired atrial and ventricular myocytes. Methods: The KATP channel current is measured by whole cell and gramicidin-perforated patch clamp recordings in 164 cultured neonate rat cardiomyocytes. Results: In whole cell recordings with 84 μmol/l ADP in pipette, spontaneous activity is significantly higher in atrium than ventricle, and EC50 for the KATP channel opener diazoxide is 0.13 μmol/l (atrium) versus 3.1 μmol/l (ventricle). With an ATP-regenerating system in pipette, EC50 for diazoxide is 19.7 μmol/l (atrium) versus 54.9 μmol/l (ventricle). In gramicidin-perforated patch recordings, atrial myocytes respond significantly to 100 nmol/l of the mitochondrial protonophore CCCP, while ventricular myocytes do not. EC50 for diazoxide is 129 μmol/l (atrium) versus >2500 μmol/l (ventricle) for myocytes exposed to CCCP, and 676 versus >2500 μmol/l, respectively, without CCCP. Conclusions: (1) KATP channels are significantly more sensitive to metabolic inhibition in atrial than ventricular myocytes. (2) Sensitivity of atrium versus ventricle to the channel opener diazoxide increases from 3:1 to ≥24:1 with ADP or metabolic inhibition. If extended to intact hearts, the results would predict a higher atrial sensitivity to ischemia, and a high sensitivity of the ischemic atrium to KATP channel openers. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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