| Autor: |
KE Tao, MAO Han, DONG Cai-hua, CHEN Shu-yuan, YU Jing-yin, ZHAO Ying-zhong, LIU Hong-yan, LIU Sheng-yi |
| Předmět: |
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| Zdroj: |
Chinese Journal of Oil Crop Sciences; 2013, Vol. 35 Issue 1, p43-47, 5p |
| Abstrakt: |
Currently, the lack of EST sequence information has limited the discovery of sesame EST - SSR primer number. All the 45 569 EST sequences of high oil content sesame species Zhongzhil were obtained from a full - length developing seeds cDNA library and assembled 32 421 unigenes. The unigenes represented about 17.428 Mb of genic sequences. Using MISA and SSR finder software to screening SSRs, 1 949 non - redundant (NR) SSRs were identified in 1 688 uniESTs. A total of 226 sequences contained more than one SSR. The trinu-cleotide repeats are the dominant types with the frequency of 74%. All of the designed 34 SSR primer pairs related to oil synthesis genes could amplify characteristic SSR fragments against 12 different oil content species, and 3 primer pairs amplified polymorphic fragments. The EST - SSR markers of sesame are highly efficiency, and should be the ideal tools for studies of genetic variation, genetic diversity, and development of genetic linkage maps of sesame. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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