| Abstrakt: |
Bacterial-type PEPC genes were cloned from four oil crops ( rape, soybean, peanut and sesame) . Their sequences of conserved domains (BOX I to BOX IV) and PEPC activity sites were characterized. The genes included Bna10093361, Bna1009749 and Bna10093360 from Brassica napus, Glyma10,g34970. 1, Glyma01g22840. 1 and Glyma02g14500. 1 from Glycine max, SIN1018296 from Sesamum indium, AhPPC5 from Arachis hypogaea. The 8 genes were commonly composed of 19 to 21 introns, and contained one unique and highly divergent insertion of about 356 to 600bp. PEPC sequences had R/KNTG in C-terminal tetrapeptide, but lacked the phosphorylation motif at N terminus. Resulls from RT - qPCR showed that Bna10093360 expressed wiih less tan 0. 1% of rapeseed BnaActin, Glyma10g34970. 1 and Glyma02g14500. 1 expressed with less than 2% of soybean GlymaActin. AhPPC5 expressed 32% to 175% of peanut AhActin, and SIN1018296 expressed 3% to 18% of sesame SINActin. The expression trends of AhPPC5 and SIN1018296 were higher at early seed developing stage and then decreased. Results indicated that the bacterial - type PEPC gene functions were widely diiferentiated. [ABSTRACT FROM AUTHOR] |
