| Autor: |
Gokmen, E, Bachier, C, Raaphorst, F M, Muller, T, Armstrong, D, LeMaistre, C F, Teale, J M |
| Předmět: |
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| Zdroj: |
Bone Marrow Transplantation; 2/15/2001, Vol. 27 Issue 4, p413, 12p |
| Abstrakt: |
It is largely unknown whether the immune repertoire can be reconstituted successfully after high-dose chemotherapy and transplantation using ex vivo expanded hematopoietic stem cell (HSC) grafts. It is critically important for the transplant outcome that immune repertoire reconstitution progresses after ex vivo expanded HSC graft transplants at least as efficiently as that seen after conventional HSC transplants. Previously, we showed that the T cell receptor V beta (TCRVB) third complementarity determining region (CDR3) diversification after ex vivo expanded bone marrow (BM) HSC graft transplants was similar to that seen after conventional peripheral blood stem cell transplants (PBSCTs). In the present study, the CDR3 diversity of the six immunoglobulin (Ig) heavy chain variable region gene (VH) families was examined in five breast cancer patients who were transplanted with ex vivo expanded BM HSCs as the only source of stem cells. For comparison, 12 healthy adults and four conventional PBSCT recipients were also studied. Using both CDR3 fingerprinting and single strand conformation polymorphism (SSCP) methodologies, it is shown that the contribution of the VH families to the overall repertoire among healthy adults is highly variable and not always proportional to VH family member size. After both ex vivo expanded HSC transplants and conventional PBSCTs, the VH CDR3 repertoires were limited in size diversity at 6 weeks post transplant. By 6 months, however, VH families displayed a repertoire diversity that was as complex as that seen in healthy adults. No difference was seen between ex vivo expanded HSC graft transplant recipients and conventional PBSCT recipients in VH repertoire diversity. In one patient there was a follow-up analysis 12 months after ex vivo expanded graft transplant, and the diversity of the VH families was maintained. In all patients, the amino acid size of the... [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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