| Autor: |
Ponterio, Eleonora, Petrizzo, Annacarmen, Bartolo, Ilaria Di, Buonaguro, Franco Maria, Buonaguro, Luigi, Maria Ruggeri, Franco |
| Předmět: |
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| Zdroj: |
Journal of Translational Medicine; 2013, Vol. 11 Issue 1, p1-7, 7p, 3 Graphs |
| Abstrakt: |
Background: Virus-like particles (VLPs) from an Italian GII.4 norovirus strain were used to investigate activation and maturation of circulating antigen presenting cells (APCs) of human origin. Methods: Peripheral blood mononuclear cells (PBMCs) isolated from five healthy subjects were pulsed ex vivo with VLPs, and stained with a set of monoclonal antibodies (MAbs) for phenotypic analysis by flow cytometry. Cytokine release in cell supernatants was investigated by ELISA. Results: Norovirus VLPs induced activation and maturation of circulating APCs derived from the five donors, as well as production of IL-6, IFN-γ and TNF-α cytokines. Conclusions: The present results suggest that VLPs can activate antigen presenting cells for an efficient induction of the adaptive immune response. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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