Quantitative Assay of Cyclin-Dependent Kinase Activity As a Sensitive Marker of Cell Proliferation in Marine Teleost Larvae.

Autor: Westerman, Mark E., Holt, G. Joan, DiMichele, Leonard
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Zdroj: Marine Biotechnology; 1999, Vol. 1 Issue 3, p297, 14p
Abstrakt: An evolutionarily conserved family of cyclin-dependent kinases (Cdks) regulates cell cycle progression in eukaryotes. Mitosis-promoting factor (MPF) is a transiently activated Cdk required for mitosis. We propose use of partially purified MPF kinase activity as a sensitive marker of cell proliferation in marine teleost larvae and describe a quantitative spin-filter assay of suc1-precipitated MPF activity. MPF extracted from embryos and larvae of red drum, Sciaenops ocellatus, phosphorylated histone H1 protein and a Cdk-specific peptide substrate. Kinetic analyses demonstrated that apparent K[SUBm] values of red drum MPF for adenosine triphosphate and the peptide substrate were 98.3 and 18.2 μM, respectively. A simple growth experiment showed that MPF activity was significantly greater in rapidly growing red drum larvae than in intermittently fed cohorts growing more slowly. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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