| Autor: |
Waterhouse, Nigel J, Finucane, Debra M, Green, Douglas R, Elce, John S, Kumar, Sharad, Alnemri, Emad S, Litwack, Gerald, Khanna, KumKum, Lavin, Martin F, Watters, Dianne J |
| Předmět: |
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| Zdroj: |
Cell Death & Differentiation; Dec98, Vol. 5 Issue 12, p1051, 11p |
| Abstrakt: |
The molecular events involved in apoptosis induced by ionizing radiation remain unresolved. In this paper we show that the cleavage of fodrin to a 150 kDa fragment is an early proteolytic event in radiation-induced apoptosis in the Burkitts' Lymphoma cell line BL30A and requires 100 µM zVAD-fmk for inhibition. Caspases-1, -3, -6 and -7 were shown to cleave fodrin to the 150 kDa fragment in vitro and all were inhibited by 10 µM zVAD-fmk. We also show that the in vitro cleavage of fodrin by calpain is inhibited by 100 µM zVAD-fmk as was the calpain-mediated hydrolysis of casein. We demonstrate that calpain is activated within 15 min after radiation exposure, concomitant with the cleavage of fodrin to the 150 kDa fragment whereas caspase-3 is activated at 2 h correlating with the cleavage of fodrin to the 120 kDa fragment. These results support a role for calpain in the early phases of the radiation-induced apoptosis pathway, upstream of the caspases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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