| Autor: |
Meredith, Gavin D., Sims, Christopher E., Soughayer, Joseph S., Allbritton, Nancy L. |
| Předmět: |
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| Zdroj: |
Nature Biotechnology; Mar2000, Vol. 18 Issue 3, p309, 4p |
| Abstrakt: |
We demonstrate a new method for the simultaneous measurement of the activation of key regulatory enzymes within single cells. To illustrate the capabilities of the technique, the activation of protein kinase C (PKC), protein kinase A (PKA), calcium-calmodulin activated kinase II (CamKII), and cdc2 protein kinase (cdc2K) was measured in response to both pharmacological or physiological stimuli. This assay strategy should be applicable to a broad range of intracellular enzymes, including phosphatases, proteases, nucleases, and other kinases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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