| Autor: |
Göttgens, Berthold, Barton, Linda M., Gilbert, James G.R., Bench, Anthony J., Sanchez, Maria-José, Bahn, Sabine, Mistry, Shailesh, Grafham, Darren, McMurray, Amanda, Vaudin, Mark, Amaya, Enrique, Bentley, David R., Green, Anthony R. |
| Předmět: |
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| Zdroj: |
Nature Biotechnology; Feb2000, Vol. 18 Issue 2, p181, 6p |
| Abstrakt: |
The SCL gene encodes a highly conserved bHLH transcription factor with a pivotal role in hemopoiesis and vasculogenesis. We have sequenced and analyzed 320 kb of genomic DNA composing the SCL loci from human, mouse, and chicken. Long-range sequence comparisons demonstrated multiple peaks of human/mouse homology, a subset of which corresponded precisely with known SCL enhancers. Comparisons between mammalian and chicken sequences identified some, but not all, SCL enhancers. Moreover, one peak of human/mouse homology (+23 region), which did not correspond to a known enhancer, showed significant homology to an analogous region of the chicken SCL locus. A transgenic Xenopus reporter assay was established and demonstrated that the +23 region contained a new neural enhancer. This combination of long-range comparative sequence analysis with a high-throughput transgenic bioassay provides a powerful strategy for identifying and characterizing developmentally important enhancers. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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