| Autor: |
Richards, Paul M., Liu, M. Maureen, Lowe, Natalie, Davey, John W., Blaxter, Mark L., Davison, Angus |
| Předmět: |
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| Zdroj: |
Molecular Ecology; Jun2013, Vol. 22 Issue 11, p3077-3089, 13p, 3 Diagrams, 3 Charts |
| Abstrakt: |
Studies on the classic shell colour and banding polymorphism of the land snail Cepaea played a crucial role in establishing the importance of natural selection in maintaining morphological variation. Cepaea is also a pre-eminent model for ecological genetics because the outward colour and banding phenotype is entirely genetically determined, primarily by a 'supergene' of at least five loci. Unfortunately, progress in understanding the evolution and maintenance of the Cepaea polymorphism stalled, partly because of a lack of genetic markers. With a view to re-establish Cepaea as a prominent model of molecular ecology, we made six laboratory crosses of Cepaea nemoralis, five of which segregated for shell ground colour ( C) and the presence or absence of bands ( B). First, scoring of colour and banding in 323 individuals found no recombination between the C and B loci of the supergene. Second, using restriction site-associated DNA sequencing ( RAD- Seq) of two parents and 22 offspring, we identified 44 anonymous markers putatively linked to the colour ( C) and banding ( B) loci. The genotype of eleven of the most promising RAD- Seq markers was independently validated in the same 22 offspring, then up to a further 146 offspring were genotyped. The closest RAD- Seq markers scored are within ~0.6 centimorgan (cM) of the C- B supergene linkage group, with the combined loci together forming a 35.8 cM linkage map of markers that flank both sides of the Cepaea C- B supergene. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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