| Autor: |
Khan, W.a., Chisholm, R., Tadayyon, S., Subasinghe, a., Norton, P., Samarabandu, J., Johnston, L.J., Knoll, J.H., Rogan, P.K. |
| Předmět: |
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| Zdroj: |
Cytogenetic & Genome Research; May2013, Vol. 139 Issue 4, p234-242, 9p, 1 Diagram, 2 Charts, 2 Graphs |
| Abstrakt: |
Despite extensive analyses on the centromere and its associated proteins, detailed studies of centromeric DNA structure have provided limited information about its topography in condensed chromatin. We have developed a method with correlative fluorescence light microscopy and atomic force microscopy that investigates the physical and structural organization of α-satellite DNA sequences in the context of its associated protein, CENP-B, on human metaphase chromosome topography. Comparison of centromeric DNA and protein distribution patterns in fixed homologous chromosomes indicates that CENP-B and α-satellite DNA are distributed distinctly from one another and relative to observed centromeric ridge topography. Our approach facilitates correlated studies of multiple chromatin components comprising higher-order structures of human metaphase chromosomes. Copyright © 2013 S. Karger AG, Basel [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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