Infection and colonization of tissues of the aphid Myzus persicae and cassava mealybug Phenacoccus manihoti by the fungus Beauveria bassiana.

Autor: Amnuaykanjanasin, Alongkorn, Jirakkakul, Jiraporn, Panyasiri, Cheerapha, Panyarakkit, Pairat, Nounurai, Prachumporn, Chantasingh, Duriya, Eurwilaichitr, Lily, Cheevadhanarak, Supapon, Tanticharoen, Morakot
Zdroj: BioControl; Jun2013, Vol. 58 Issue 3, p379-391, 13p
Abstrakt: Histopathogenesis of living insects of Myzus persicae Sulzer (Hemiptera: Aphididae) and Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) by Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreales) was monitored from penetration through insect death. Important events in aphids included fungal penetration of the integument of the less-resistant leg intersegmental membrane and invasion of natural openings, formation of hyphal bodies in live aphids by three days post-inoculation (PI), and extensive hyphal colonization of the two leg segments closest to the insect body at death of the aphids. Confocal microscopy of green fluorescent protein-labeled B. bassiana in live mealybugs indicated the fungus penetrated the host through the legs and mouthparts. The fungus was scarce in live mealybugs at 1-5 days PI, formed hyphal bodies by six days PI, and growth was limited to parts of dead hosts at 6-7 days PI. In dead mealybugs, hyphal bodies were near solid tissue. Blastospores were in the hemolymph. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index