COUNTERCURRENT CHROMATOGRAPHIC SEPARATION OF WATER-SOLUBLE VITAMINS BY CROSS-AXIS COIL PLANET CENTRIFUGE USING AN ION-PAIR REAGENT WITH POLAR TWO-PHASE SOLVENT SYSTEM.

Autor: Shinomiya, Kazufusa, Yoshida, Ken, Kabasawa, Yozo, Ito, Yoichiro
Předmět:
Zdroj: Journal of Liquid Chromatography & Related Technologies; 10/1/2001, Vol. 24 Issue 17, p2615-2623, 9p, 2 Charts
Abstrakt: Countercurrent chromatographic separation of water-soluble vitamins, such as riboflavin sodium phosphate, cyanocobalamin, riboflavin, pyridoxine hydrochloride, and thiamine nitrate was performed using the cross-axis coil planet centrifuge equipped with a pair of eccentric coil assemblies at off-center position. The solvent system used in the present studies was prepared by adding 1.5% of 1-octanesulfonic acid sodium salt, as an ion-pair reagent, to the polar two-phase solvent system composed of 1-butanol and aqueous 0.15 M monobasic potassium phosphate. The resolution was remarkably improved as compared with that obtained in our previous studies. Overall experimental results revealed that the cross-axis coil planet centrifuge is useful for the separation of extremely hydrophilic and ionic compounds, such as water-soluble vitamins, by adding a suitable ion-pair reagent to the polar two-phase solvent system. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje