| Autor: |
Shu Li, Ma, XiaoXin, Li Ma, Cuicui Wang, He, YuanQi, Yu, ZhiJuan |
| Předmět: |
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| Zdroj: |
Journal of Experimental & Clinical Cancer Research (17569966); 2013, Vol. 32 Issue 1, p1-6, 6p, 1 Black and White Photograph, 3 Charts, 3 Graphs |
| Abstrakt: |
Objectives: To investigate the role of HER-2/neu-mediated COX-2/P450arom signal in estrogen-dependent endometrial carcinoma. Methods: The recombinant eukaryotic expression vector, pcDNA3.1-HER-2/neu, was constructed and transfect to Ishikawa endometrial carcinoma cells. The expression of COX-2 and P450arom in transfected cells were detected by real-time PCR and western blotting. The levels of estrogen in cell supernatants were detected by ELISA. Results: Over-expression of HER-2/neu in transfected cells was confirmed by real-time PCR and western blotting. The levels of autocrine estrogen in transfected cells was significantly increased which combination with the enhancement of COX-2 and P450arom expression in transfected cells. Conclusion: HER-2/neu induced the improvement of autocrine estrogen in endometrial carcinoma cell through triggering the COX-2/P450arom signal. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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